Hsueh-Chia Chang's Biography

Hsueh-Chia Chang, Bayer Professor of Chemical and Biomolecular Engineering, University of Notre Dame, Interim Chief Technology Officer, Aopia Biosciences

Over 300 publications, with 18,000 citations and h index of 76. He has confounded a startup Aopia Biosciences in 2020 on purification of extracellular vesicles and currently serves as its interim CTO.

Droplet Emulsion Generation based on Electrokinetics: Digital PCR and Cell Encapsulation

Wednesday, 9 October 2019 at 09:00

Add to Calendar ▼2019-10-09 09:00:002019-10-09 10:00:00Europe/LondonDroplet Emulsion Generation based on Electrokinetics: Digital PCR and Cell EncapsulationSELECTBIOenquiries@selectbiosciences.com

We report a new microdroplet generation technology that uses AC electric field rather than hydrodynamic shear to generate droplets. Intricate flow-rate tuning of both phases is hence unnecessary and massive parallelization is now possible because of the absence of hydrodynamic cross-talk. The bulky and expensive precision micropump is replaced by a simple and miniature AC source that can be driven by a battery. With a less viscous oil phase and a properly tuned AC frequency, we are able to eliminate electro-coalescence and Rayleigh fission. Monodispersed droplets with CV less than 3% and with a size range from 10 to 100 microns can be generated at a high throughput of nearly 1000 Hz per nozzle. Moreover, the size of the droplets can be easily adjusted by simply tuning the voltage or the frequency. One hundred to one million droplets and, with parallelization, more than 1 billion droplets can be generated in 30 minutes. This large range allows high-dynamic range digital PCR and conformal cell encapsulation.

Add to Calendar ▼2019-10-07 00:00:002019-10-09 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics 2019: Emerging Themes, Technologies and Applications Track "A"SELECTBIOenquiries@selectbiosciences.com

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