High Throughput Error-Correction Code DNA SequencingThursday, 14 November 2019 at 15:30  Add to Calendar ▼2019-11-14 15:30:002019-11-14 16:30:00Europe/LondonHigh Throughput Error-Correction Code DNA SequencingMicrofluidics and Organ-on-a-Chip Asia 2019 in Tokyo, JapanTokyo, JapanSELECTBIOenquiries@selectbiosciences.comEliminating errors in next-generation DNA sequencing has proved challenging. I am going to talk about our newly developed error-correction code (ECC) sequencing, a method to greatly improve sequencing accuracy by combining fluorogenic sequencing-by-synthesis (SBS) with an information theory–based error-correction algorithm. ECC embeds redundancy in sequencing reads by creating three orthogonal degenerate sequences, generated by alternate dual-base reactions. This is similar to encoding and decoding strategies that have proved effective in detecting and correcting errors in information communication and storage. We showed that, when combined with a fluorogenic SBS chemistry with raw accuracy of 98.1%, ECC sequencing provides single-end, error-free sequences up to 200 bp. ECC approaches should enable accurate identification of extremely rare genomic variations in various applications in biology and medicine. |
Add to Calendar ▼2019-11-14 00:00:002019-11-15 00:00:00Europe/LondonMicrofluidics and Organ-on-a-Chip Asia 2019Microfluidics and Organ-on-a-Chip Asia 2019 in Tokyo, JapanTokyo, JapanSELECTBIOenquiries@selectbiosciences.com
