Rapid Development of Small-Molecule Producing Bacteria using Metabolite Sensors and RecombineeringTuesday, 11 March 2014 at 14:45 Add to Calendar ▼2014-03-11 14:45:002014-03-11 15:45:00Europe/LondonRapid Development of Small-Molecule Producing Bacteria using Metabolite Sensors and RecombineeringSingle Cell Analysis Europe 2014 in Berlin, GermanyBerlin, GermanySELECTBIOenquiries@selectbiosciences.com We developed individual metabolite sensors to monitor cytosolic concentrations of L-lysine, L-histidine, L-arginine and further amino acids in C. glutamicum and E. coli. This methodology enables a number of novel FACS-based applications, including high-throughput isolation of individual producers from large mutant populations or in-vivo site-directed saturation mutagenesis using recombineering. |