Dr. Sagawa started his career as a scientist for reagent development in life science research area at Bio Products Development Center at Takara Shuzo Co. Ltd. (currently, a part of Takara Bio, Inc.). Subsequently he worked on new product development and new business initiation. Dr. Sagawa led technology and product development projects, in areas such as T cell expansion methods, and insertion sites and clonality analysis system of gene modified cells for gene and cell therapy. Several technologies developed in these projects have been applied to clinical studies. After the acquisition of Clontech Laboratories, Inc. by Takara Bio, Dr. Sagawa joined Clontech in the US as Vice President of R&D. He was at Clontech from January 2006 to March 2012 where he developed a comprehensive R&D strategy and strategic project integration plan. Dr. Sagawa has also held an endowed associate professor at Kyoto Prefectural University of Medicine from 2012 to 2013 and an associate professor at United Graduate School of Agricultural Science, Kagoshima University from 2005 until 2010. Dr. Sagawa joined JSR in 2013. He has been working on business expansion in Life Science Research and In Vitro Diagnostics. He has worked on the establishment and execution of strategic project plans for Technology and Business Development in Life Science Research and In Vitro Diagnostics, especially in Liquid Biopsy area. Hiroaki Sagawa has a Ph.D. from Osaka Prefecture University.
New Tools for Exosome Isolation and Analysis: ExoCap Kit for Serum, Plasma & Cell Culture
Monday, 21 March 2016 at 15:00
Add to Calendar ▼2016-03-21 15:00:002016-03-21 16:00:00Europe/LondonNew Tools for Exosome Isolation and Analysis: ExoCap Kit for Serum, Plasma and Cell CultureSELECTBIOenquiries@selectbiosciences.com
Research in circulating tumor cells, circulating cell-free DNA, extracellular RNA, and Extracellular Vesicles (EVs) have been gaining popularity as a promising non-invasive diagnostic approach. With this backdrop, JSR has focused on the development of EV capture methodologies, keeping in mind future potential uses for EVs in non-invasive diagnostics at the point of care. In this session, a description of a selective capture method of EVs as a potential platform for non-invasive diagnostics will be provided by introducing ExoCapTM, a magnetic beads based isolation and enrichment method of exosomes from various body fluids and cell culture supernatants. ExoCap consists of Magnosphere magnetic beads particles coupled with antibodies that recognize antigens on the exosome surface, a washing buffer, and a reagent that releases the captured exosomes for analysis. The antibodies against CD9, CD63, CD81, and EpCAM were specifically selected for this kit. ExoCap can easily separate exosomes, without ultracentrifuge or any special equipment. A sample amount of 0.1mL is sufficient. In addition, it is an animal free system which is superior to other methods for Mass Spec analysis. Moreover, this method enables non-destructive purification of exosomes. ExoCap can also be applied to beads based Flow Cytometry immediately after the capture process with 50 µL of samples. To confirm exosomal isolation from diverse body fluids (such as human serum, plasma) and cell culture media, exosomes were examined by western blot, RT-qPCR, particle size distribution measurement, Flow Cytometry, and scanning transmission electron microscopy (TEM). Exosomes isolated by ExoCap had a lipid bilayer membrane, showed a particle size distribution around 100nm, and expressed tetraspanin molecules.
Add to Calendar ▼2016-03-21 00:00:002016-03-22 00:00:00Europe/LondonExosomes and Microvesicles: Research, Biomarker Cargo and Therapeutic PotentialSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2016-03-21 15:00:002016-03-21 16:00:00Europe/LondonNew Tools for Exosome Isolation and Analysis: ExoCap Kit for Serum, Plasma and Cell CultureSELECTBIOenquiries@selectbiosciences.com
