Daniel Chiu,
A. Bruce Montgomery Professor of Chemistry,
University of Washington
Daniel T. Chiu is currently the A. Bruce Montgomery Professor Chemistry, Endowed Professor of Analytical Chemistry, and Professor of Bioengineering at the University of Washington. He is a member of the University of Washington’s Center for Nanotechnology, Neurobiology and Behavior Program, and the Cancer Consortium of the Fred Hutchinson Cancer Research Center. He has authored more than 180 publications and is the inventor on over 40 issued patents. Dr. Chiu obtained a B.A. in neurobiology and a B.S. in chemistry at the University of California, Berkeley in 1993, and a Ph.D. in chemistry from Stanford University in 1998.
Multi-Parametric High-Throughput Flow-Based Analysis of Single Extracellular Vesicles
Tuesday, 14 December 2021 at 16:00
Add to Calendar ▼2021-12-13 16:30:002021-12-13 17:30:00Europe/LondonHigh-Purity and High-Sensitivity Rare-Cell Isolation with Aliquot SortingExtracellular Vesicles (EVs): Technologies and Biological Investigations in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
We have developed a multi-parametric high-throughput flow-based method for the analysis and sorting of individual extracelluar vesicles (EVs), which are highly heterogeneous and comprise a diverse set of surface protein markers as well as intra-vesicular cargoes. Yet, current approaches to the study of EVs lack the necessary sensitivity and precision to fully characterize and understand the make-up and the distribution of various EV subpopulations that may be present. In fact, most current EV isolation methodologies, including differential centrifugation, affinity/immuno-magnetic isolation, polymer-based precipitation, size-based exclusion, can be prone to contaminations and cannot resolve single EVs. Our new technology platform is able to immune-phenotype EVs and quantify proteins associated with EVs, at the single-EV level, with high precision and throughput.
High-Purity and High-Sensitivity Rare-Cell Isolation with Aliquot Sorting
Monday, 13 December 2021 at 16:30
Add to Calendar ▼2021-12-13 16:30:002021-12-13 17:30:00Europe/LondonHigh-Purity and High-Sensitivity Rare-Cell Isolation with Aliquot SortingExtracellular Vesicles (EVs): Technologies and Biological Investigations in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
This presentation describes the high-sensitivity isolation of rare
cells, including circulating tumor cells and fetal cells, using
fluorescence activated aliquot sorting. Here, a blood sample is divided
into nanoliter volumes or aliquots, then analyzed and sorted based on
the presence or absence of a rare cell. By performing two rapid, on the
millisecond timescale, back-to-back sorting, we also demonstrate
isolation of rare cells with high purity. Finally, we drastically
increased the number of nucleated cells sorted by first concentrating
peripheral blood mononuclear cells from whole blood, which translated
into an equivalent blood volume analyzed by over an order of magnitude,
from 8mL to over 100mL per experiment.
Add to Calendar ▼2021-12-13 00:00:002021-12-15 00:00:00Europe/LondonExtracellular Vesicles (EVs): Technologies and Biological InvestigationsExtracellular Vesicles (EVs): Technologies and Biological Investigations in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2021-12-13 16:30:002021-12-13 17:30:00Europe/LondonHigh-Purity and High-Sensitivity Rare-Cell Isolation with Aliquot SortingExtracellular Vesicles (EVs): Technologies and Biological Investigations in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com
