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Revealing the Diversity of EVs with Single Vesicle Analysis

Thursday, 25 February 2021 at 13:00

Add to Calendar ▼2021-02-25 13:00:002021-02-25 14:00:00Europe/LondonRevealing the Diversity of EVs with Single Vesicle AnalysisExtracellular Vesicles (EV)-Exosomes: Diagnostics, Delivery and Therapeutics in Virtual ConferenceVirtual ConferenceSELECTBIOenquiries@selectbiosciences.com

Extracellular vesicles (EVs) appear to be involved in many physiological processes and have attracted great attention as potential diagnostic and therapeutic targets. The realization of this potential has been hampered by the lack of analytical methods with the necessary specificity, sensitivity, and quantitative rigor needed for robust hypothesis testing, biomarker development, and EV engineering. New analytical tools with improved specificity and sensitivity are revealing the diverse constituents of biofluid preparations that had been previously referred to as “exosomes” and “microvesicles,” and are forcing a re-evaluation of both methods and nomenclature. Single vesicle analysis represents the ultimate level of EV analysis, and we have been using single vesicle flow cytometry (vFC) to count, size, and measure the molecular cargo on individual EVs at a large scale. I will review the state of EV analytics, highlight critical elements of single EV analysis, and share insights into EV diversity that have emerged from the application of quantitative analytical methods to the study of EVs.