Zoltan Varga's Biography

Zoltan Varga, Biological Nanochemistry Research Group, Research Centre for Natural Sciences (RCNS)

Dr. Zoltan Varga received his M.Sc. in Engineering Physics and Ph.D. in Chemistry from Budapest University of Technology and Economics. Dr. Varga is a group leader at the Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Budapest, Hungary. The aim of his research group is to connect traditional colloid chemistry with new challenges in biology and medicine. He holds an appointment as work package leader in the METVES II project, “Standardisation of concentration measurements of extracellular vesicles for medical diagnoses” funded by the European Metrology Programme for Innovation and Research. His lab currently focusing on the development of novel synthetic reference materials and isolation and characterization techniques for EV research.

Single Particle Detection of Extracellular Vesicles and Native SARS-CoV-2 Virions by Microfluidic Resistive Pulse Sensing

Friday, 26 February 2021 at 09:30

Add to Calendar ▼2021-02-26 09:30:002021-02-26 10:30:00Europe/LondonSingle Particle Detection of Extracellular Vesicles and Native SARS-CoV-2 Virions by Microfluidic Resistive Pulse SensingExtracellular Vesicles (EV)-Exosomes: Diagnostics, Delivery and Therapeutics in Virtual ConferenceVirtual ConferenceSELECTBIOenquiries@selectbiosciences.com

Microfluidic resistive pulse sensing (MRPS) is the nanoscale implementation of the Coulter principle in a microfluidic cartridge. As such, MRPS is a non-optical, single-particle detection method to measure the size distribution and concentration of nanoparticles. MRPS can be used to characterize extracellular vesicles (EVs). Enveloped viruses such as SARS-CoV-2 are spherical biological nanoparticles in the hundred nanometer size range, therefore MRPS is the method of choice for their characterization. In this study, MRPS was used to measure the size distribution of various EVs and SARS-CoV-2. The analysis of the supernatant of infected Vero cells indicated an envelope diameter of approximately 80 nm for the SARS-CoV-2 virions, which is in good agreement with previous cryo-EM studies. The detection of more than ten thousand virions made it possible to determine the size distribution of SARS-CoV-2 and paves the way for the MRPS-based detection of COVID-19 infection.

Add to Calendar ▼2021-02-25 00:00:002021-02-26 00:00:00Europe/LondonExtracellular Vesicles (EV)-Exosomes: Diagnostics, Delivery and TherapeuticsExtracellular Vesicles (EV)-Exosomes: Diagnostics, Delivery and Therapeutics in Virtual ConferenceVirtual ConferenceSELECTBIOenquiries@selectbiosciences.com

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