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SELECTBIO Conferences 3D-Culture, Organoids & Organ-on-a-Chip Europe 2021

Silvia Bolognin's Biography

Silvia Bolognin, Research Scientist, University of Luxembourg

Dr. Bolognin is a pharmaceutical chemist by training and holds a Ph.D. in tissue and grafting engineering obtained at the University of Padova, Italy. After graduation, she completed her post-doctoral training in several laboratories: USA, Italy, and Luxembourg. She is now a senior research scientist at the Luxembourg Centre for Systems Biomedicine (LCSB). Since her bachelor thesis, she has been studying neurodegenerative mechanisms associated with Alzheimer’s Disease, which she has later extended to Parkinson’s Disease. In 2014 she also joined the field of stem cell technologies to optimize new and more relevant in vitro models to study disease mechanisms and to develop new therapeutics.

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3D Cultures to Study Parkinson’s Disease

Tuesday, 29 June 2021 at 13:00

Add to Calendar ▼2021-06-29 13:00:002021-06-29 14:00:00Europe/London3D Cultures to Study Parkinson’s Disease3D-Culture, Organoids and Organ-on-a-Chip Europe 2021 in Rotterdam, The NetherlandsRotterdam, The

Parkinson’s disease (PD) is the second most common neurodegenerative disease affecting the aging population. Despite tremendous research efforts in the course of more than a century, the cause of the disease is still unknown. Our lab focuses on the optimization of advanced human in vitro models to understand the molecular basis underlying PD. We have established the cultivation of PD patient-specific neurons, derived from induced pluripotent stem cells carrying the LRRK2-G2019S mutation, in 3D microfluidics. In contrast to conventional 2D cultures, this 3D approach reveals robust endophenotypes. High-content imaging data show decreased dopaminergic differentiation and branching complexity, altered mitochondrial morphology, and increased cell death in LRRK2-G2019S neurons compared to controls without using stressor agents. Pushing forward the optimization of a model recapitulating the human brain tissue, the cultivation of midbrain brain organoids was also established in microfluidic devices. PD organoids show fewer dopaminergic neurons compared to organoids derived from healthy subjects. These data support the use of 3D models to study complex cellular mechanisms underlying PD and allow for testing the effect of disease-modifying compounds using human-derived material.

Add to Calendar ▼2021-06-28 00:00:002021-06-30 00:00:00Europe/London3D-Culture, Organoids and Organ-on-a-Chip Europe 20213D-Culture, Organoids and Organ-on-a-Chip Europe 2021 in Rotterdam, The NetherlandsRotterdam, The