Dr. Sirenko is an established Cell Biologist and Imaging Specialist who is an international expert in development of assays with complex cell-based models for research and drug discovery. She is a Senior Scientist at Molecular Devices where she has worked extensively with High Content Imaging and its application to analysis of novel cell systems. Dr. Sirenko currently leads a group of scientists and engineers developing methods and new tools for automation of 3D cell models – including organoids and organ-on chips – for modeling cancer, neurotoxicity, cardiac diseases and toxicology. She is also actively involved in establishing collaborations in the areas of cell culture, microfluidics, and image analysis. She has over 15 years of industry experience, holds a PhD in Biochemistry/Biophysics, and has authored more than 36 scientific papers in the fields of cell biology, toxicology, and biotechnology.
Automation of 3D Organoid Culture with Monitoring, Imaging and Analysis of Complex Biological Effects
Tuesday, 29 June 2021 at 14:00
Add to Calendar ▼2021-06-29 14:00:002021-06-29 15:00:00Europe/LondonAutomation of 3D Organoid Culture with Monitoring, Imaging and Analysis of Complex Biological Effects3D-Culture, Organoids and Organ-on-a-Chip Europe 2021 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
There is an unmet need for cell-based model systems that better resemble human biology to improve pre-clinical selection of drug candidates. 3D cell models representing various tissues were successfully used for studying complex biological effects, tissue architecture, and functionality. While complexity of 3D models remains a hurdle for the wider adoption in research and drug screening, significant progress has been made in developing tools for automated cell culture, monitoring, and image analysis. We will describe an integrated cell culture and a high-content imaging process that allows automated culture, monitoring, maintenance, and characterization of growth and differentiation of organoids and stem cells, as well as testing the effects of various compounds. We will discuss the methods for automation of three complex workflows: maintenance and monitoring of iPSC cell culture, culturing and testing toxicity effects in lung organoids, and monitoring of the development of intestinal organoids. The automated integrated system included ImageXpress Confocal HT.ai High-Content Imaging System, (IXM-C HT.ai confocal imaging system), automated CO2 incubator (Wave STX44), a liquid handler (Biomek i7), as well as an automation robotic system and software. Described workflow demonstrates the useful tools for increased throughput and automation in organoid assays and compound screening, and analysis methods and descriptors for gaining more information from complex assays ?useful for studying disease phenotypes and compound effects.
Add to Calendar ▼2021-06-28 00:00:002021-06-30 00:00:00Europe/London3D-Culture, Organoids and Organ-on-a-Chip Europe 20213D-Culture, Organoids and Organ-on-a-Chip Europe 2021 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2021-06-29 14:00:002021-06-29 15:00:00Europe/LondonAutomation of 3D Organoid Culture with Monitoring, Imaging and Analysis of Complex Biological Effects3D-Culture, Organoids and Organ-on-a-Chip Europe 2021 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
