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SELECTBIO Conferences Exosomes and Microvesicles

Vasilis Toxavidis's Biography

Vasilis Toxavidis, Resource Director of BIDMC/HSCI Flow Cytometry Core, Beth Israel Deaconess Medical Center

After obtaining a degree in Podiatric Medicine from University of Wales, Vasilis’s first position was at South County Podiatric Associates, USA.
From there, he transitioned to Academic research at Roger William’s Hospital (a Brown University teaching hospital) in the Pathology Department.
In 2002, Vasilis joined a growing BIDMC Flow Cytometry Core in Boston, MA. There his research interests grew to stem cell biology, landing him as a crucial member of the Harvard Stem Cell Institute.
Current Research Interests: The BIDMC/HSCI current research interests revolve around Small Particle Detection and cutting edge technologies.

Vasilis Toxavidis Image

Beckman Coulter Cytoflex Violet SSC: An alternative to FSC PMT or Fluorescence in the Detection of Extracellular Vesicles

Monday, 23 March 2015 at 14:00

Add to Calendar ▼2015-03-23 14:00:002015-03-23 15:00:00Europe/LondonBeckman Coulter Cytoflex Violet SSC: An alternative to FSC PMT or Fluorescence in the Detection of Extracellular

The interest in the identification and detection of submicron particles has increased in recent years.  The ability to study them has been hindered by available techniques to measure particles at sizes below 1um.  Flow Cytometry has become an important tool in EV research with instrumentation being developed to identify particles at the submicron level. Instrumentation such as cytometers optimized to improve light scattering collection and image cytometers.  However, most equipment designed for the detection of EVs is expensive and complex.  Hardware enhancements have focused around the development of the FSC PMT.  While the FSC PMT enhancements have proven to enable the flow cytometer to detect particles <200nm in size, the instrumentation is not practical for all lab settings. In this comparison study, it has been shown that the Cytoflex is compatible to the results obtained from the AstriosEQ, NanoView, and Gallios.  The ability to resolve and distinguish the populations as effectively as its counterparts, has proven the Cytoflex Violet SSC to be a viable alternative to the FSC PMT to detect EVs.

Add to Calendar ▼2015-03-23 00:00:002015-03-24 00:00:00Europe/LondonExosomes and