| Conferences \ Circulating Biomarkers World Congress 2015 \ Exosomes and Microvesicles \ Agenda \ John Tigges |
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Beckman Coulter Cytoflex Violet SSC: An alternative to FSC PMT or Fluorescence in the Detection of Extracellular VesiclesMonday, 23 March 2015 at 14:00  Add to Calendar ▼2015-03-23 14:00:002015-03-23 15:00:00Europe/LondonBeckman Coulter Cytoflex Violet SSC: An alternative to FSC PMT or Fluorescence in the Detection of Extracellular VesiclesSELECTBIOenquiries@selectbiosciences.comThe interest in the identification and detection of submicron particles has increased in recent years. The ability to study them has been hindered by available techniques to measure particles at sizes below 1um. Flow Cytometry has become an important tool in EV research with instrumentation being developed to identify particles at the submicron level. Instrumentation such as cytometers optimized to improve light scattering collection and image cytometers. However, most equipment designed for the detection of EVs is expensive and complex. Hardware enhancements have focused around the development of the FSC PMT. While the FSC PMT enhancements have proven to enable the flow cytometer to detect particles <200nm in size, the instrumentation is not practical for all lab settings. In this comparison study, it has been shown that the Cytoflex is compatible to the results obtained from the AstriosEQ, NanoView, and Gallios. The ability to resolve and distinguish the populations as effectively as its counterparts, has proven the Cytoflex Violet SSC to be a viable alternative to the FSC PMT to detect EVs. |
Add to Calendar ▼2015-03-23 00:00:002015-03-24 00:00:00Europe/LondonExosomes and MicrovesiclesSELECTBIOenquiries@selectbiosciences.com
