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SELECTBIO Conferences Exosomes and Microvesicles

John Tigges's Biography

John Tigges, Flow Cytometry Science Center Director, Beth Israel Deaconess Medical Center

John Tigges is the Director of the Flow Cytometry Science Center at Beth Israel Deaconess Medical Center. He has been working in the field of Flow Cytometry for over 25 years and has extensive experience in cell sorting, multiparametric panel design, and small particle detection. His latest research in the detection of extracellular vesicles has allowed him to co-chair a nano-flow cytometry standardization group and become a member of the ISEV/ISAC/ISTH EV working group. He has worked with CytoU and ISAC Education Task force to advance the flow cytometry knowledge base and is a member of the Instruments for Science task force which assists in bringing Flow Cytometry equipment and education to underserved communities.

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Beckman Coulter Cytoflex Violet SSC: An alternative to FSC PMT or Fluorescence in the Detection of Extracellular Vesicles

Monday, 23 March 2015 at 14:00

Add to Calendar ▼2015-03-23 14:00:002015-03-23 15:00:00Europe/LondonBeckman Coulter Cytoflex Violet SSC: An alternative to FSC PMT or Fluorescence in the Detection of Extracellular

The interest in the identification and detection of submicron particles has increased in recent years.  The ability to study them has been hindered by available techniques to measure particles at sizes below 1um.  Flow Cytometry has become an important tool in EV research with instrumentation being developed to identify particles at the submicron level. Instrumentation such as cytometers optimized to improve light scattering collection and image cytometers.  However, most equipment designed for the detection of EVs is expensive and complex.  Hardware enhancements have focused around the development of the FSC PMT.  While the FSC PMT enhancements have proven to enable the flow cytometer to detect particles <200nm in size, the instrumentation is not practical for all lab settings. In this comparison study, it has been shown that the Cytoflex is compatible to the results obtained from the AstriosEQ, NanoView, and Gallios.  The ability to resolve and distinguish the populations as effectively as its counterparts, has proven the Cytoflex Violet SSC to be a viable alternative to the FSC PMT to detect EVs.

Add to Calendar ▼2015-03-23 00:00:002015-03-24 00:00:00Europe/LondonExosomes and