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SELECTBIO Conferences Microfluidics for Circulating Biomarkers Summit 2019

Microfluidics for Circulating Biomarkers Summit 2019 Agenda

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Thursday, 10 October 2019


Redbud LabsCartridge-Ready Isolation and Concentration of Extracellular Vesicles
Richard Spero, CEO, Redbud Labs

Despite decades of innovation, there is no accepted standard for isolation of circulating biomarkers in microfluidic systems. This creates a problem for developers of new biomarker assays and liquid biopsy tests. Traditional sample prep methods such as ultracentrifugation, affinity sorting with magnetic beads, or micro-filtration are acceptable for use in a discovery environment. However, these methods do not translate effectively to microfluidic cartridges, so promising assays must be completely re-implemented when moving from the bench to an integrated system. We present our work on a high-performance sample prep method for circulating biomarkers that translates easily from the bench to a microfluidic cartridge.


Using exRNAs to Diagnose Diseases and Injuries of the Central Nervous System
Kendall Van Keuren-Jensen, Associate Professor, Translational Genomics Research Institute, United States of America

A variety of RNAs are secreted into biofluids by every cell type, packaged within extracellular vesicles (EVs) or bound to proteins. exRNAs present in multiple biofluids can act as potential prognostic or predictive biomarkers of a wide range of diseases and injuries.


Lydia SohnKeynote Presentation

Title to be Confirmed.
Lydia Sohn, Professor of Mechanical Engineering, University of California-Berkeley, United States of America


Microfluidic Cellular Engineering System For Precision Immunotherapy
Mei He, Assistant Professor, University of Kansas, United States of America

Cells as the smallest units of the material body are taking tremendous roles in maintaining human body functions. Modifying cells as the factory to produce essential biomolecules are of substantial economic and scientific interest. In addition to the production of proteins, metabolites, and enzymes, engineering and production of exosomes are emerging for developing precision immunotherapy agents and personalized medicine. We will report novel microfluidic approaches for manipulating cellular functions in producing tailored extracellular vesicles and exosomes for advancing the cancer immunotherapy.


Integrated Microfluidic Devices For Enrichment and Detection of Circulating Biomarkers
Hyo-Il Jung, Professor, Yonsei University, Korea South

The precise measurement or isolation of circulating biomarkers (CB) can serve as a powerful tool for cancer prognosis, diagnosis of minimal residual disease, assessment of tumor sensitivity to anticancer drugs, and personalization of anticancer therapy. The microfluidic device has a very strong advantage in comparison with   various modalities for the enrichment and separation of the CB in that it can be operated in a continuous manner minimizing any chance of loss. Over the past decade, our group has been working on various types of microfluidic-based enrichment and detection methods for the down-stream analysis of CBs. In this talk, I will present some of new discoveries and discuss critical concerns and future directions for the research.


Hsueh-Chia ChangKeynote Presentation

Multiplex Liquid Biopsy Platform for Fractionation of Heterogeneous Vesicles and Precision Analyses of Their RNA Cargo
Hsueh-Chia Chang, Bayer Professor of Engineering, University of Notre Dame, United States of America

Extracellular RNAs in blood are promising circulating biomarkers.  However, as they can be easily degraded, extracellular RNAs must form complexes with proteins or are encapsulated by liposomes, exosomes or microvesicles to remain stable.  As a result, stable RNA biomarkers, like mRNA and miRNA, are carried by a heterogeneity of nanocarriers in blood with wide size, electrophoretic mobility and isoelectric point distributions.  Due to their different biogenesis, these carriers have specific surface proteins and are often selective in their choice of RNA cargoes.  To offer more precise quantification of the circulating RNA biomarkers, we have developed a suite of microfluidic modules that can fractionate the carriers by size, surface proteins and isoelectric point.  Size-based separation is achieved by an asymmetric nanoporous membrane whose conic pores reduce hydrodynamic resistance and vesicle fusion.  Extensive rinsing can be carried out to remove free-floating contaminating proteins like albumin.  We also developed several lysing modules that can lyse the vesicles and dissociate the complexes non-chemically, such that low-yield extraction can be eliminated as an intermediate step.  We integrate these modules with our membrane sensor, digital PCR module and nanopore sensor for RNA identification and quantification to achieve an integrated multiplexed platform for precise profiling of RNA cargoes in fractionated carriers.  Some of the upstream modules can also be integrated into a pretreatment platform for NGS.


Sehyun ShinKeynote Presentation

Title to be Confirmed.
Sehyun Shin, Professor & Director, Nano-Biofluignostic Engineering Research Center, Korea University and Anam/Guro Hospital of Korea University, Korea South

Venue: Coronado Ballroom 7


Microfluidic Strategies for Isolation and Enrichment of Circulating Biomarkers
Hyo-Il Jung, Professor, Yonsei University, Korea South

Cancer-derived materials can be obtained from various body fluids (liquid biopsy) using a minimally invasive manner and provide information for disease progression in real-time. Circulating biomarkers (CBs) analyzable through such liquid biopsy include circulating tumor cells (CTCs), exosomes, circulating cell-free DNA (cfDNA), miRNA, and proteins. Microfluidic devices for the isolation and detection of CBs have been actively developed because they can be utilized for the fields of diagnosis, prognosis, and treatment. However, owing to the rarity and heterogeneity of CTCs, CTC research should have overcome technical hurdles. Exosomes and cfDNA are being highlighted as new target materials because they also have genetic information on cancers. In this presentation, several microfluidic technologies for circulating biomarkers will be introduced and discussed for the future directions in cancer screening, detection, and diagnostics.


Hsian-Rong TsengKeynote Presentation

Title to be Confirmed.
Hsian-Rong Tseng, Professor, Crump Institute for Molecular Imaging, California NanoSystems Institute, University of California-Los Angeles, United States of America


Near-Patient, Automated Platform for Rapid Microfluidic Extraction of Circulating Nucleic Acids from Milliliter Volumes of Whole Blood
Maiwenn Kersaudy-Kerhoas, Associate Professor, School of Engineering and Physical Sciences, Heriot-Watt University, United Kingdom

Extracellular plasma circulating cell-free nucleic acids (CNAs) are promising clinical biomarkers but their measurement remains time-consuming, technically challenging and expensive. For CNAs to have an impact on healthcare, a key challenge to overcome is the development of rapid and reliable low-cost sample preparation. There is an acknowledged issue around CNAs stability in the presence of hemolysis, and few solutions for fast and robust extraction at the site of blood draw. We demonstrate a microfluidic system able to perform the extraction of circulating miRNAs from several milliliters of whole blood in a single disposable fluidic cartridge, on a fully automated platform, delivering a stable elution of CNAs in less than 45 minutes. The biological characterization of the eluates include qPCR, fluorometric and spectrophotometric analysis, and automated electrophoresis for fragment analysis. This platform enables the standardization of sample preparation at the point of blood draw and in resource limited settings and could aid the introduction of CNAs-based assays into routine clinical practice.

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Add to Calendar ▼2019-10-10 00:00:002019-10-11 00:00:00Europe/LondonMicrofluidics for Circulating Biomarkers Summit 2019Microfluidics for Circulating Biomarkers Summit 2019 in Coronado Island, CaliforniaCoronado Island,