Separation and Detection Agenda

Registration

Polymeric Monolithic Materials and Liquid Chromatography
Brett Paull, Professor, University of Tasmania, Australia

This presentation will highlight some of the latest and innovative applications of polymer monolithic materials in separation science. The presentation will detail new approaches to the fabrication and modification of monolithic substrates and their further modification with structured and selective surface chemistry/biochemistry.

Coffee Break and Networking in the Exhibition Hall

The Power of Liquids - Separations using Liquid-liquid Technologies
Ian Garrard, Manager, Brunel University, United Kingdom

The presentation will describe some of the unique advantages of liquid-liquid technologies, such as the ability to accept particulates or to flow continuously, giving case examples as necessary.

Lunch and Networking in the Exhibition Hall

Poster Viewing Session

Nano-Agglomerated Stationary Phases for Solid Phase Extraction
Damian Connolly, Senior Researcher, Dublin City University, Ireland

The preparation and application of silica monoliths with a dense surface coating of nanoparticles (iron oxide or gold) for enrichment of phosphopeptides or glycoproteins respectively shall be discussed. Furthermore, the preparation of continuous gold films upon capillaries or stationary phases will also be shown.

Novel Developments in Separation Modeling Technology
Imre Molnar, CEO, Molnar-Institute, Germany

QbD and Design Space are new terms for HPLC user. Continual improvement and the establishment of robust methods based on solid science can only be achieved in a short time if modeling technologies are applied.

Coffee Break and Networking in the Exhibition Hall

Plastic Antibodies: A Promising Tool for the Purification and Detection of Biomolecules
Borje Sellergren, Head, Technical University of Dortmund, Germany

The talk will describe our efforts to develop molecularly imprinted polymers (MIPs), in soluble form termed "plastic antibodies", for use as capture agents in peptide, protein or lipid analysis. I will emphasize the added value of these receptors over their biological counterparts such as the recognition of peptidic biomarkers under nonphysiological conditions e.g. acetonitrile-buffer mixtures or in denaturing media.

Rapid and Sensitive Identification of Bacillus and Clostridium Spore Coat Proteins
Chris de Koster, Professor, University of Amsterdam, Netherlands

In this presentation we will highlight the principles of our protein isolation and identification methods to detect covalently bound proteins that are candidates for biomarkers and vaccines at the outer spore coat layer of endospores from Bacillus subtilis and the pathogenic micro-organisms Bacillus cereus and Clostridium difficile.

Homeostasis? Variability of VOC Profiles in Humans and the Implications for Marker Discovery in the Volatile Domain
Paul Thomas, Professor, Loughborough University, United Kingdom

Any individual’s metabolism changes significantly throughout the course of a typical day. How does hunger, stress and digestion affect VOC profiles in human? This presentation describes the research undertaken to characterise such complexity, in the VOC profiles of saliva, skin and breath.

Drinks Reception

Probing Plants and Surfaces by Ambient Mass Spectrometry: Overview and Recent Applications
Teris Beek, Associate Professor, Wageningen University, Netherlands

After briefly introducing ambient MS, some new exciting developments are highlighted. Several DART-MS and DESI-MS applications from the author’s group related to natural products chemistry and modified organic surfaces are presented.

In-line LCxLC Hyphenation by Machine Vision Controlled Solvent Evaporation
Jan-Willem Schoonen, Research Associate, Leiden University, Netherlands

We developed a machine vision controlled solvent evaporation interface and droplet sample transfer device to increase the separation capacity and analyte detectability for multidimensional liquid chromatography systems to aid complex sample analysis.

Coffee Break and Networking in the Exhibition Hall

Microfluidic Systems for the Analysis of Mass-limited Samples
Han Gardeniers, Professor, University of Twente, Netherlands

Microfluidic technology allows accurate handling of liquid volumes in the nl to µl range, in miniaturized systems with integrated in-situ monitoring elements. This development is especially relevant for the Life Sciences, but also offers opportunities for process analytical technology. Several device examples will be discussed, including HPLC on a chip and microfluidic NMR and IR probes.

Lunch and Networking in the Exhibition Hall

Poster Viewing Session

Too Big for a Small World? Intact Protein Analysis by Nano-UHPLC-MS/MS
Mark Parkin, Lecturer, Kings College London, United Kingdom

Investigating intact protein analysis by LC-MS/MS, thought the use of a sensitive and robust nano-UHPLC-MS/MS method. Quantification of intact, prohibited, IGF-I in sport using very small volumes of human serum is demonstrated.

Recent Progress in Core-shell and Embedded Nanoparticle Technology for Biomarker Separation with Amperometric Sensing at a Boron Doped Diamond Electrode.
Jeremy Glennon, Professor, University College Cork, Ireland

New superficially porous or core shell silica particle technology for liquid chromatography is described, which allows nanoscale control over shell thickness, and high efficiency separations. The enhancement of separation selectivity for important low molecular biomarkers using embedded gold and silica nanoparticles is illustrated using capillary electrophoresis, where the sensitivity is also enhanced using amperometric detection.

Close of Conference, Coffee Break and Networking in the Exhibition Hall