Ryuji Morizane,
Assistant Professor,
Harvard Medical School; Visiting Scholar, Wyss Institute
Dr. Morizane is a Principal Investigator at Massachusetts General Hospital, an Assistant Professor at Harvard Medical School, an Affiliated Faculty at Harvard Stem Cell Institute, and a Visiting Scholar at Wyss Institute. He has pioneered research in stem cell differentiation and kidney organoids. He directs research groups focused on kidney regenerative medicine, genome editing in stem cells, and kidney disease modeling. His research has been recognized internationally, and he has received various awards including NIH Director’s New Innovator Award in 2019.
Recreating Kidney Organogenesis in vitro with Human Pluripotent Stem Cells
Thursday, 20 August 2020 at 13:30
Add to Calendar ▼2020-08-20 13:30:002020-08-20 14:30:00Europe/LondonRecreating Kidney Organogenesis in vitro with Human Pluripotent Stem Cells2D-to-3D Culture and Organoids 2020 in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com
We have developed an efficient, chemically defined protocol for differentiating human pluripotent stem cells into multipotent nephron progenitor cells (NPCs) that can form kidney organoids. By recapitulating metanephric kidney development in vitro we generate SIX2+SALL1+WT1+PAX2+ NPCs with 80-90% efficiency within 8-9 days of differentiation. NPCs form kidney organoids containing epithelial nephron-like structures expressing markers of podocytes, proximal tubules, loops of Henle and distal nephrons in an organized, continuous arrangement that resembles the nephron in vivo. The organoids express genes reflecting many transporters seen in adult metanephric-derived kidney, enabling assessment of transporter-mediated drug nephrotoxicity. Stromal cells are also generated with the presence of PDGFRBeta+ fibroblasts/pericytes, and CD31+ endothelial cells. This kidney differentiation system can be used to study mechanisms of human kidney development. Repetitive injury to tubular cells causes interstitial fibroblast expansion with characteristics of myofibroblasts, indicating kidney organoids can be used to model kidney fibrosis in vitro. Polycystic kidney disease (PKD) patient-derived organoids exhibit cystic phenotypes. Hence the generated kidney organoids are effective tools to study genetic disorders of the kidney as well as mechanisms of kidney injury and fibrosis. Microphysiological platforms in vitro facilitate kidney organoid vascularization and maturation, which may lead to the development of functional bioengineered kidneys in the future.
Add to Calendar ▼2020-08-19 00:00:002020-08-20 00:00:00Europe/London2D-to-3D Culture and Organoids 20202D-to-3D Culture and Organoids 2020 in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2020-08-20 13:30:002020-08-20 14:30:00Europe/LondonRecreating Kidney Organogenesis in vitro with Human Pluripotent Stem Cells2D-to-3D Culture and Organoids 2020 in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com
