Ionita Ghiran,
Assistant Professor of Medicine,
Beth Israel Deaconess Medical Center
Dr. Ghiran received his MD from the “Iuliu Hatieganu” Medical School University, Cluj Napoca in Romania, before completing his postdoctoral training at Beth Israel Deaconess Medical Center in Boston where he is currently an Assistant Professor in the Department of Medicine at the Beth Israel Deaconess Medical Center (BIDMC)/Harvard Medical School in Boston, MA. Dr. Ghiran’s focus in on understanding the regulation of extracellular vesicles originating from red cells, and the role of the red cell-derived miRNAs in normal and pathological conditions. Recently, Dr. Ghiran and his colleagues described the significant impact of the circadian rhythm in the number and protein composition of circulating blood extracellular vesicles, as well as in the origin and quantity of various extracellular miRNAs species.
The Role of Red Blood Cell-derived Microparticles in Cardiac Remodeling
Tuesday, 24 March 2015 at 15:30
Add to Calendar ▼2015-03-24 15:30:002015-03-24 16:30:00Europe/LondonThe Role of Red Blood Cell-derived Microparticles in Cardiac RemodelingSELECTBIOenquiries@selectbiosciences.com
Advances in the management of acute myocardial infarction (MI), notably the extensive use of reperfusion therapies have led to a significant decline in early mortality. However, surviving patients with myocardial damage continue to suffer substantial morbidity and mortality from its sequelae. The adverse mechanical remodeling that occurs subsequent to an MI, hallmarks of which include fibrosis and hypertrophy, underlies the development of heart failure (HF). The intimate mechanisms responsible for progression towards HF are not currently well understood. During normal and inflammatory conditions, such as MI, complement activation targets the plasma membrane of nearby cells, especially red blood cells (RBCs), generating large quantities of EVs. We have found that: (i) circulating RBC-derived EVs reach a maximum early in the morning hours, and (ii) RBC-derived EVs are enriched in miR-451, a regulator of mTOR and AMPK pathways. Moreover, our co-culture experiments shown that RBC-derived EVs efficiently fuse with and transfer the EV miRNA content to both endothelial and cardiac cells. Following the fusion of the RBC-derived EVs to endothelial cells, the RBC-derived Ago-2:miRNA-451 complex effectively targeted several mRNAs species such as, ATF-2, CAV-2 and S1RP-2, all of which contain miR451 recognition site. The net result was a significant down-regulation of the levels of targeted mRNA following 12 hours post-EVs incubation. Thus, our in vitro results suggest a novel mechanisms responsible for cardiac remodeling, specifically the unforeseen role of circulating RBCs and RBC-derived EVs in this process.
Add to Calendar ▼2015-03-23 00:00:002015-03-24 00:00:00Europe/LondonExosomes and MicrovesiclesSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2015-03-24 15:30:002015-03-24 16:30:00Europe/LondonThe Role of Red Blood Cell-derived Microparticles in Cardiac RemodelingSELECTBIOenquiries@selectbiosciences.com
