MetaboMeeting 2015 Agenda

Coffee and Registration

Welcome and Introduction
Andrew Nicholls, Director & Secretary, Metabolic Profiling Forum, United Kingdom
Jules Griffin, Director, Metabolic Profiling Forum University of Cambridge, United Kingdom
Roy Goodacre, Professor of Biological Chemistry, University of Manchester, United Kingdom

Coffee and Networking in Exhibition Hall

Towards Reliable Mass Spectrometry Methods for Metabolomics Research
Alan Race, Research Scientist, National Physical Laboratory, United Kingdom

We present results from studies probing the performance of MALDI, DESI, PADI and LESA for a range of analytes. Metrology investigations into sampling mechanisms, optimum ion source configurations and technique fundamentals will be discussed.

Label-free Raman Imaging of Living Mammalian Cells - A Valuable New Tool for Investigating Complex Cellular Systems
Katherine Hollywood, Research Associate, Manchester University, United Kingdom

A current area of research that is gaining increasing interest is the field of single cell analysis. Raman microspectroscopy provides a novel, label-free and non-invasive approach for the analysis of cellular perturbations within single cells in real-time.

A Novel Lipid Screening Platform Allowing a Complete Solution for Lipidomics Research
Baljit Ubhi, Lead Staff Scientist, Metabolomics & Lipidomics Applications, AB Sciex, United Kingdom

A Novel Lipid Screening Platform Allowing a Complete Solution for Lipidomics Research

Lunch and Networking in Exhibition Hall

Free Workshop
Metabolomics - From Data to Knowledge
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MetExplore
Fabien Jourdan, Research Scientist, INRA-MetaboHub, France

This talk will present MetExplore, a freely available web server making possible the analysis and visual inspection of genome scale metabolic networks. Graph based methods to extract sub-networks based on metabolic profiles will also be reviewed in this presentation.

Data-driven, Non-targeted Metabolomic Approaches Using High Resolution Mass Spectrometry and Advanced Machine Learning Algorithms
Manfred Beckmann, Research Fellow, University of Wales Aberystwyth, United Kingdom

Non-targeted metabolomics is combined with advanced machine learning algorithms and high performance computing clusters, to elucidate metabolic changes in a range of biological systems. Discriminatory features are elucidated with chromatography and tandem mass spectrometry (MSn), without the need for extensive targeted studies.

Coffee and Networking in Exhibition Hall

Translating Big Data from HR Imaging MS Data into Molecular Knowledge
Theodore Alexandrov, Team Leader, EMBL Heidelberg, Germany

Imaging Mass Spectrometry promises to localize hundreds metabolites in tissue sections with a cellular resolution. We will present our pipeline for molecular annotation of millions of imaging MS signals and how we can control our newly-developed False Discovery Rate.

Using High Resolution Mass Spectrometry (HRMS) Approaches to Fight Food Fraud: Don’t Get Stung by Your Manuka Honey!
Joanne Connolly, Omics Business Development, Waters, United Kingdom

Understanding, deconvoluting and identifying the biochemical profile of a food sample of interest can help provide manufacturers and regulators with key information in the fight against fraud. The identification of a MS derived biochemical “fingerprint” is an important tool to understanding the ultimate question of “What is normal?” This presentation will describe the analytical and data processing workflows for evaluating a pilot study of different unifloral honeys.

Building Comprehensive Statistical Workflows for the Large-scale Analysis of Human Cohorts: Application to the Study of Urine Metabolome Physiological Variations with Age, Body Mass Index and Gender
Etienne Thevenot, Research Scientist, CEA, France

Robust and comprehensive statistical workflows are critical for the analysis of large-scale cohorts by LC-HRMS. Here we present the combination of uni- and multivariate strategies to the study of physiological variations of the urine metabolome.

Poster Session A (Drinks will be served)

Coffee and Registration

Fat, Sugar and Metabolomics – Understanding How Diabetes Arises at the Population Level
Jules Griffin, Director, Metabolic Profiling Forum University of Cambridge, United Kingdom

In this presentation I will describe how metabolomics and lipidomics are being applied to large cohorts in conjunction with genome wide association studies to better define genes associated with type 2 diabetes and understanding the underlying mechanisms.

Metabolic Perturbations Triggered by Western Diet under Impaired Fatty Acid Oxidation: Getting Clues from Metabolomics Studies in PPAR-alpha Null Mice
Manuel Pazos, Postdoc, University of Córdoba, Spain

The present research evaluates for the first time by metabolomics the metabolic outcomes of Western diet on the animal model of impaired fatty acid oxidation PPAR-alpha null mice.

Urine Metabolomic Profiling to Identify Biomarkers of a Flavonoid-rich and Flavonoid-poor Fruits and Vegetables Diet in Adults
Maria Ulaszewska, Post-doc Researcher, Fondazione Edmund Mach, Italy

The present study aims to investigate the dose dependent effects of consuming diets enriched in flavonoid-rich and flavonoid-poor fruits and vegetables on the urine metabolome of adults who had a = 1.5 fold increased risk of cardiovascular diseases (CVD).

Coffee and Networking in Exhibition Hall

Identification of Biosynthetic Function of Genes in Plants and their Consequences in Insects – A Metabolite Profiling Approach Driven by Automatic Compound Identification
Heiko Neuweger, Dr, Bruker Daltonik GmbH, Germany

An efficient software based work-flow for metabolite profile screening and automatic compound identification is presented for the discovery of gene-mediated glycosylation in the HGL-DTG biosynthetic pathway in tobacco and their consequences in the herbivore M. sexta.

Chemotyping in the Field: The Application of High Resolution Metabolomics to Study the Miscanthus Metabolome
Thomas Wilson, Student, Aberystwyth University, United Kingdom

High resolution mass spectrometry fingerprinting methods have been used to explore the metabolome of the bio-energy grass Miscanthus, in order to maximise the understanding of the plant metabolome and add value with a bio-refining chain.

Lunch and Networking in Exhibition Hall

Free Workshop
Increased Throughput, Sensitivity and Confidence for Metabolomics Research Using a New Tribrid™ Orbitrap Mass Spectrometer Workshop
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Differences of Metabolic Perturbations between Thai Jasmine Rice and its Brown Planthopper (BPH)-resistant Isogenic Lines During BPH Infestation using 1H NMR and GC-MS Approaches
Umaporn Uawisetwathana, Student, Queen's University Belfast, United Kingdom

Differences of metabolic perturbations between Thai Jasmine rice and its BPH-resistant isogenic lines during BPH infestation were investigated using 1H NMR and GC-MS-based metabolomics. From metabolomic differences, the susceptible and resistant varieties seemed to employ different pathways to fight against BPH infestation.

Longitudinal Profiling of Amino Acids Metabolism with Respect to Markers for Disturbed Glucose and Insulin Homeostasis in Obese Children
Christian Hellmuth, Post-Doc, Ludwig-Maximilians-University of Munich, Germany

HOMA, amino acids and acylcarnitines were analyzed longitudinal in obese children from the Obeldicks 1-year intervention cohort. This longitudinal study revealed a positive association between the aromatic AA tyrosine to IR, rather than BCAA.

Clinical Untargeted Metabolomics to Study the Effects of Glucocorticoids Administration and their Interaction with Insulin
Riccardo Di Guida, PhD Student, University of Birmingham, United Kingdom

The role of glucocorticoids in human metabolism is still unclear. Two untargeted UPLC-MS metabolomics studies were performed on human serum in order to assess the effects of glucocorticoids administration and their interaction with insulin.

Coffee and Networking in Exhibition Hall

Finding Novel Enzymatic Activities Linked to Human Diseases using Bioinformatics and Untargeted Metabolite Profiling
Charandeep Singh, PhD Student, Luxembourg Center for Systems Biomedicine, Luxembourg

I will present a combined bioinformatics and experimental pipeline to discover new enzymatic activities relevant to human diseases, with, as an illustration, our recent molecular identification of a conserved sugar phosphotransferase.

Tissue Metabonomic Study of Giant Cell Tumor of Bone
Francisco Javier Martínez-López, Student, Instituto Politecnico Nacional, Mexico

The untargeted metabonomic study of Giant Cell Tumour in bone tissue shows differences between tumour tissues and healthy bone samples. The study performed by a 1H-NMR approach, the data submitted to multivariate statistics and enrichment-pathway analyses.

Predictive Ability of Blood Plasma Carnitines in Cardiovascular Diseases
Zsuzsanna Ament, Investigator Scientist, MRC Human Nutrition Research, United Kingdom

We used machine learning and multivariate statistics to identify Carnitines which differentiated in the different patient groups.

Poster Session B (Drinks will be served)

Organised Walking Tour: The Eagle, Discovery of DNA, Turing and JJ Thompson

Conference Dinner, The Hall, Kings College, Cambridge

Coffee and Registration

Enhancing Metabolomics: Shining Light on Metabolism
Roy Goodacre, Professor of Biological Chemistry, University of Manchester, United Kingdom

Surface enhanced Raman scattering provides quantitative analyses and LC-SERS allows absolute quantification of target analytes at levels lower or comparable to LC-MS

Metabolomic Composition and Spatial Metabolite Distribution in the Human Lens
Vadim Yanshole, Researcher, International Tomography Center, Russian Federation

Metabolomic profiles of normal and cataractous human lenses and spatial metabolite distribution are established inside the human lens with the use of high-frequency 1H NMR and high-resolution mass spectrometry with HPLC separation methods.

Mitochondrial Metabolomics: Identifying Early in-vivo Biochemical Markers of Mitochondrial Toxicity
James R Armitage, Senior Scientist, GSK, United Kingdom

There are no easily accessible biomarkers to monitor compound-mediated effects on mitochondria in-vivo. A known mitochondrial toxin was employed to identify metabolites that change in advance or absence of a clinical effect to identify sensitive in-vivo indicators of mitochondrial function.

Coffee and Networking in Exhibition Hall

Targeting Resistance to Proteasome Inhibitor Therapy: A Metabolomics Approach
Celia Berkers, Assistant Professor, Utrecht University, Netherlands

Treatment with proteasome inhibitors is often hampered by the occurrence of resistance. Here, the metabolic mode of resistance to such drugs is elucidated using a metabolomics approach, revealing a potential role for nutrient starvation in the treatment of resistant tumours.

Metabolomics Platform to Elucidate the Impact of Dietary Fiber in the Human Gut Metabolome Determined in vivo and in an in vitro Colon Simulator
Santosh Lamichhane, PhD Fellow, Aarhus University, Denmark

The aim of present study is to demonstrate the usefulness of metabolomics to elucidate the impact of dietary fiber on the gut metabolome. Examples from a human intervention and an in-vitro colon simulator study will be presented.

Lunch and Networking in Exhibition Hall

Free Workshop
Removing the Data Processing Bottleneck: Move Efficiently from Data to Structure to Pathway with NEW Thermo Scientific™ Compound Discoverer™ 2.0 and mzCloud Workshop
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Lipidomics Associations of Phospholipids with Obesity and Insulin Resistance in Young Adults
Sebastian Rauschert, PhD Student, Ludwig-Maximilians Universität, Germany

Mass spectrometry based metabolomics revealed polar lipids associated with obesity and different mechanisms for the development of insulin resistance depending on BMI status in 20 years old obese and non-obese subjects taking part at the Western Australian Pregnancy Cohort (Raine) Study.

Coffee and Networking in Exhibition Hall

A Non-targeted Metabolomics Analysis to Study Longitudinal Metabolic Changes Following a Burn Injury in Adults
Warwick Dunn, Lecturer in Metabolomics, University of Birmingham, United Kingdom

A non-targeted UPLC-MS study of adult and elderly subjects following a burn trauma has been performed. The data show three distinct metabolic phases during the recovery period and identify targets for nutritional or therapeutic interventions to improve recovery and outcome.

Prizes and Meeting Close