Advances in qPCR & dPCR Agenda

Registration & Light Breakfast

Opening Welcome - Mikael Kubista, Professor/Founder, TATAA Biocenter AB and the Institute of Biotechnology, Czech Academy of Sciences

Discovering Circulating MicroRNA Biomarkers Using a Highly Controlled qPCR Workflow
Lihan Zhou, Co Founder/Chief Technology Officer, MiRXES Pte Ltd, Singapore

Coffee Break and Networking in Exhibition Area

Tube-strip digital PCR: fast, easy and Clarity
Johnson Ng, Founder And CEO, JN Medsys Pte Ltd, Singapore

Ultra-Sensitive Screening and Monitoring of Tumor Pathology, Relapse and Drug Resistance Using Tissue and Liquid Biopsy (ctDNA/ctRNA) Methods
Yafei Liu, Head, Commercial Operation, RainDance Technologies, Singapore

Current and emerging applications of droplet digital PCR in Cancer Research
Eli Mrkusich, Global Digital Applications Specialist, Bio-Rad Laboratories, United States of America

Color-Coded Finicky Molecular Beacons for Multiplex Digital PCR
Fred Kramer, Professor, New Jersey Medical School Rutgers University, United States of America

Technology Spotlight:
Integrating Genetic Analysis Through Multiple Platforms on the Cloud
Peter Norster, Regional Market Development Manager, Thermo Fisher Scientific

Lunch Break and Networking in Exhibition Area

Absolute copy number from the statistics of the quantification cycle Cq in replicate qPCR experiments
Joel Tellinghuisen, Professor of Chemistry, Vanderbilt University, Vanderbilt University, United States of America

Data Analysis of droplet digital PCR with Generalised Linear Mixed Models
Olivier Thas, Professor in Biostatistics & Honorary Professor, Ghent University, Belgium

Coffee Break and Networking in Exhibition Area

Quantitative PCR as a Discrete Dynamical System
Steven Smith, Professor, City of Hope Beckman Research Institute, United States of America

Landmark of Real Time PCR in Differential Expression Analysis of Genes in Some Crop Plants
Dev Pandey, Associate Professor, Birla Institute of Technology, India

End of Day One

Registration & Light Breakfast

Opening Welcome - Fred Kramer, Professor, New Jersey Medical School, Rutgers University

Improved qPCR performance using internally quenched probes
Mark Behlke, Vice President/Chief Scientific Officer, Integrated DNA Technologies Inc, United States of America

Coffee Break and Networking in Exhibition Area

Selfie-qPCR : accurate expression measurement without reference gene
Petar Podlesniy, Postdoctoral Research Associate, Consejo Superior De Investigaciones Científicas, Spain

Microfluidic PCR instrumentation for high-throughput gene expression profiling
Christopher Hayes, Researcher, University of Limerick, Ireland

In the following presentation, an instrument for automated, high-throughput, low volume amplification of DNA in a droplet-based micro?uidic system is described.

Microfluidic RT-qPCR for Point-of-Care Liver Toxicity Assessment
Geok Soon Lim, PhD Student, Nanyang Technological University, Singapore

Lunch Break and Poster Session with Prize Award in Exhibit Hall

Genomic quantification of isolated single cells on a microfluidic device based on hot cell-direct PCR
Izumi Kubo, Professor, Soka University, Japan

Gene detection and genomic quantification of isolated single cells on an original microfluidic device for the high-throughput isolation of single cells were performed. An original detection system composed of a fluorescent microscope and an automatic image acquisition was designed for the detection of PCR product of single cells. The system was applied to detect amplicon of novel PCR/RT-PCR of the genes using fluorescent probe. The genomic quantification of the isolated cells are described in this presentation.

Expanding the Scope of Systems Biology Using Single Cells
David Ruff, Visiting Senior Research Fellow/ Principal Scientist , Oxford University/Fluidigm Corporation, United States of America

A simple, sensitive in solution proximity ligation assay workflow for quantifying proteins in complex samples
Mark Shannon, Senior Staff Scientist, Thermo Fisher Scientific, United States of America

Coffee Break and Networking in Exhibition Area

PANEL DISCUSSION - ”Global Standardization – what can we do and what is being done to make results obtained across the world comparable” Panel Chair is Mikael Kubista, Professor/Founder, TATAA Biocenter AB, Sweden

Close of Conference